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An update version of the Cotton Oligonucleotide Microarray (v2) has been released.
The original Cotton Oligonucleotide Microarray (v1) was composed of 12,006 IDT
oligonucleotides derived from an assembly of more than 180,000 Gossypium ESTs
sequenced from 30 cDNA libraries. Subsequently, approximately 9,000 additional
oligonucleotide probes have been designed bringing the total number of oligonucleotide
probes to approximately 21,000. Sequences from fifty-two cDNA libraries were collected
from 14 different research groups across the globe. These libraries were constructed
from a variety of tissues and organs under a range of conditions, including drought
stress and pathogen challenges, and represents allopolyploid cotton as well as its two
diploid progenitors. Most cDNA libraries (28) were derived from G. hirsutum and
were relatively small (from 576 to 8,643 ESTs) with one exception. The ovule cDNA
library from TM-1 cultivar was recently created and 32,789 new ESTs sequences were
generated. Collectively, these G. hirsutum EST collections comprised 64% of the
total used in the assembly. The remaining ESTs were derived from three deeply sampled
cDNA libraries generated from the two diploids (one library from 7-10 dpa fiber of
G. arboreum and two libraries of G. raimondii), comprising 15% and 23%
of the total number of ESTs, respectively. The G. arboreum fiber library was
normalized to remove highly redundant transcripts (Arpat et al., 2004), while the
G. raimondii cDNA libraries were not normalized because their redundancy was so
low, perhaps due to the heterogeneous tissue sources used for RNA isolation (whole
flowers and whole seedlings). To browse or query the ESTs that make up these libraries,
go to PAVE in the EST libraries section of the website.
Development of this microarray was funded as part of two separate Plant Genome Program
awards from two separate National Science Foundation awards with Jonathan Wendel and
Z. Jeffery Chen as the Principle Investigators, respectively. The microarrays are
printed at the
University of Washington's core facility on Corning epoxy slides. These microarrays
are publicly available through the
Ordering page.
If you do not have in-house hybridization facilities, you may also be interested in the
hybridization services offered by Washington University. These services are independently
provided by the Washington University Microarray Core Facility and not the investigators
who developed the microarray. You will need to work with WashU for hybridization pricing,
RNA shipments, hybridization scheduling, data retrieval, and data analysis.
The Cotton Oligonucleotide Microarray (v2) GAL file is available for
download.
Please note that we have recently (5/10/2007) updated our probe Ids, adding a unique number identifier
as the primary Id. The gal file has been updated as well. This has been done as an aid to tracking related Ids as sequence
assemblies are updated.
An Id conversion table is also available.
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Microarrays
