Overview
This project adds breadth to the existing public cotton
EST resources,
based on an additional ~45,000 cDNA sequence reads two very
different cDNA libraries from a diploid
D-genome G. raimondii.
The first cDNA library (GR__Ea) was derived from RNA isolated from
whole seedlings which had produced their first true leaves.
The second cDNA library (GR__Eb) was derived from RNA isolated from
bulked -3, -2, -1, 0, +1, +2, and +3 floral developmental stages (images below).
Our sequencing efforts resulted in 33,671, and 35,061 (68,732 total)
high quality reads, respectively. The sequences complement the
A- and
AD-genome cotton ESTs
already available. All of the cDNA clones and
D-genome derived sequences
(also available in Genbank) are available upon
request.
Sequence data is also available from our custom EST browser named
PAVE
(Program for Assembling and Viewing ESTs).
PAVE allows the user to query assemblies of individual libraries or all the libraries together, and search the contigs
or ESTs with a user supplied sequence. PAVE is currently under development and the assemblies are subject to change without
notice (though the current assembly is usually good for a couple weeks at a time).
| Cotton floral developmental stages |
| -3 |
-2 |
-1 |
0 |
1 |
2 |
|
|
|
|
|
|
We welcome your comments and suggestions.
Microarrays
